rat anti β actin  (Proteintech)

Journal: iScience

Article Title: Single-cell transcriptomic analyses reveal angiogenic vascular responses to chronic cerebral hypoperfusion

doi: 10.1016/j.isci.2026.115331

Figure Lengend Snippet: Tip cells show enhanced function in angiogenesis with an upregulation in Apln after VD (A) Volcano plot showing a total of 1,472 up- (right) and downregulated (left) DEGs (|log2 FC| > 1 and Bonferroni-adjusted p value <0.05) of tip cells versus other EC subclusters in ACAS brains. (B) Gene Ontology (GO) enrichment analysis of DEGs identified in (A). Five major classes of GO terms were identified. Top genes of each plotted GO term are shown in the right. Dot colors representing the significance level (−log10(adjusted p value)) and dot sizes indicating the number of genes in each category. (C) UMAP plot showing expression of Apln and Aplnr in ACAS and sham cells. (D) UMAP plots depicting the co-expression visualization of Apln and Aplnr in ECs. The colors represent scaled expression levels. (E) Representative immunostaining images of Apln (red), tip cell marker CD93 (green), endothelial cell marker CD31 (magenta), and DAPI nuclear staining (blue) in the brain 35 days after ACAS. The Apelin + CD93 + CD31 + tip cells in white squares are enlarged and 3D reconstructed to show colocalization. (F) Representative images of western blots for Apln in brain 35 days after ACAS or sham operation. (G) Quantification of protein levels of Apln. β-actin was used as a loading control. n = 4–7/group. Student’s t test. Data are shown as mean ± SEM. ∗ p < 0.05. Scale bars are indicated in images.

Article Snippet: The primary antibodies used include Apln (1:500, Cat# PA5-114860; Invitrogen, Carlsbad, CA) and β-actin (1:5000, Cat# MAB8929; R&D systems, Minneapolis, MN).

Techniques: Expressing, Immunostaining, Marker, Staining, Western Blot, Control